Researcher's Profile

YUNG-LING LEE (李永凌)

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(Leo)

Research Outline      2014-12-10 14:09:46

 

The goal of our laboratory is to explore the genetic regulation, immunological development, prognostic factors and pharmacogenetics in allergic diseases. We have tried to identify novel loci of dermatitis and asthma and to use the molecular biology approaches to understand mechanisms underlying the associations. We hope these works could be applied in clinical settings for improving personalized prevention and treatment protocols of allergic diseases.

Our current research projects include:

  1. To explore genetic susceptible loci for multiple subphenotypes of asthma. Deep sequencing and functional genetic studies will be applied to verify the findings. To study pharmacogenetics and pharmacogenomics of asthma control medications (b2-agonist, corticosteroid and leukotriene inhibitor). The therapeutic effects of these drugs have been explored under both prospective and retrospective clinical designs and the prediction models will be built for asthma personalized therapy. We would also try to develop diagnostic kits for screening drug efficacy in future translational work.
  2. To study the roles of skin in immunological function. Keratinocytes and fibroblasts are major components of human skin. They provide the physical barrier against outside pathogens, protect the body by producing anti-microbial peptides, and interact with immune cells. We are interested in the interactions between skin and immune system and their relationships with skin inflammation and skin allergy.
  3. To understand whether glycosylation process regulates the interactions between skin and immune system, and whether glycosylation process would influence the pathogenesis of skin inflammation and skin allergy. We have applied biochip analysis in skin tissues from patients with allergic dermatitis, psoriasis or contact dermatitis. Based on these results, we have chosen some glycosyl-transferases, such as Fut8 and GalNAcT6, as study targets. Besides, we also investigate the functional roles of the downstream glycoproteins in the skin inflammatory mechanisms.

本實驗室致力於探索過敏疾病的發生、免疫進展、預後及藥物基因體學。我們找出與皮膚炎、氣喘有關的基因,並利用基因體醫學和分子免疫學等研究平台來瞭解相關分子機轉,期望可以找出有臨床應用價值的分子,逐步邁向過敏疾病個人化治療與預防的終極目標。

目前我們重點研究的方向有:

  1. 尋找氣喘各種次表現型的基因區段,進行功能性基因體研究,並針對多種氣喘藥物進行藥物遺傳學研究,期望可以開發篩檢療效之基因檢測套組,逐步開展臨床轉譯工作。
  2. 研究皮膚在免疫功能上扮演的重要角色。皮膚中主要組成是角質細胞(keratinocyte)和纖維母細胞(fibroblast),在結構上能阻隔病源菌、產生抗菌蛋白,還能與免疫細胞作用,我們探討這二種細胞與免疫系統的交互作用、以及皮膚發炎與過敏之間的關係。
  3. 探討醣基化(glycosylation)過程調控皮膚與免疫系統,以及是否和皮膚發炎與過敏的致病機轉有關。我們利用異位性皮膚炎、乾癬、接觸性皮膚炎患者皮膚切片檢體進行生物晶片分析,目前挑選Fut8和GalNAcT6等醣化酵素作為研究標的,另外,我們也探討皮膚發炎反應路徑中相關醣蛋白(glycoprotein)醣基化程度,與其分子訊號傳遞之間的關聯。